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The HPLC Analysis of Aflatoxins in Raw Peanuts with Sep-pak Cleanup

Authors: W. Jeffrey Hurst , Kevin P. Snyder , Robert A. Martin

  • The HPLC Analysis of Aflatoxins in Raw Peanuts with Sep-pak Cleanup

    ARTICLES

    The HPLC Analysis of Aflatoxins in Raw Peanuts with Sep-pak Cleanup

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Abstract

An HPLC method for the determination of aflatoxins is described. The aflatoxins are extracted with aqueous acetone and interfering compounds precipitated with CuCO3. After defatting, the aflatoxins are extracted into CH2Cl2 for cleanup with silica Sep-pak® which eliminates other interfering compounds. The resulting extract is then treated with TFA to form the hemiacetal derivatives prior to final HPLC analysis. Data indicated that the method exhibits excellent accuracy and precision. In addition, it is time and solvent conservative.

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Keywords: HPLC, aflatoxin, raw peanuts

How to Cite:

Hurst, W. & Snyder, K. & Martin, R., (1984) “The HPLC Analysis of Aflatoxins in Raw Peanuts with Sep-pak Cleanup”, Peanut Science 11(1), p.21-23. doi: https://doi.org/10.3146/i0095-3679-11-1-7

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Published on
01 Jan 1984
Peer Reviewed
License
Creative Commons Attribution-NonCommercial 4.0
Hershey Foods Corporation, Analytical Research Group, 1025 Reese Avenue, P. O. Box 805, Hershey, PA 170330805