1Research supported by the USDA, ARS, SAA, and the Univ. of Georgia, Griffin, GA 302231797.
Embryo axes explants from deteriorated seed of peanut (Arachis hypogaea L.) were incubated at a 16 hr photoperiod at 26 C on an MSB5 medium containing MS salts, B5 vitamins, 20 g/L sucrose, and 8 g/L agar. Five to 8-wk-old plants regenerated from embryonic axes were transplanted to Jiffy pots in the greenhouse. Thirty-two samples of deteriorated seed between 2 and 32 yr old were evaluated. Significant differences in organogenesis were observed between different seed accessions. Shoots and roots were recovered from 74 and 36%, respectively, of embryonic axes explants. In the same experiment, seed producing plants were recovered from 14- to 31-yr-old deteriorated seed of E-2, PI 275704, Macrocarpa, G33, G34, G64, Strain No. 5, TMV 3, PI 290608, PI 295981, Sekelembwe, PI 298879, PI 337300, and PI 371850, by in vitro rescue of embryonic axes, while no plants were recovered from seed of 31 seed accessions germinated in the field. The in vitro rescue of embryonic axes can significantly increase the recovery of germplasm from deteriorated seed of peanut.
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Keywords: Arachis hypogaea, peanuts, Tissue Culture, Germination, seed longevity, seed viability
How to Cite:
Morris, J. & Dunn, S. & Pittman, R., (1995) “Plant Recovery from Embryonic Axes of Deteriorated Peanut Seed for Germplasm Renewal¹”, Peanut Science 22(1), p.66-70. doi: https://doi.org/10.3146/pnut.22.1.0015