1This research was supported in part by state funds allocated to the Georgia Experiment Station, in part by the Peanut CRSP, U.S. AID Grant Number DAN-4048-G-SS-206500, and in part by the EMBRAPA-Purdue University Contract Program.
Peanut seeds were tested by the enzyme-linked immunosorbent assay (ELISA) for peanut stripe virus without affecting their viability. A portion of cotyledon (0.02-0.05 g) was removed from the end of the seed opposite the radicle and triturated in an antigen buffer. Virus was frequently detected in extracts of cotyledons and embryos but seldom in the testa. Portions of one infected seed in ten could be detected. Correlations among ELISA, infectivity assays, and growing out tests were obtained. The ELISA technique has the advantage of detecting individual infected seed allowing the elimination of the infected ones. Identified infected seed can be used for preservation of virus cultures.
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Keywords: Arachis hypogaea, ELISA, peanut stripe virus, seed assay, seed transmission
How to Cite:
Demski, J. & Warwick, D., (1986) “Testing Peanut Seeds for Peanut Stripe Virus¹”, Peanut Science 13(1), p.38-40. doi: https://doi.org/10.3146/i0095-3679-13-1-12