This study evaluated various specimen preparation techniques for light microscopy (LM) and scanning electron microscopy (SEM). Main conclusions from this study were:
(i) Critical point drying (CPD) was preferred over freeze drying for SEM of whole or large pieces of specimens. However, CPD did not offer any additional advantage over air drying for SEM of thin (14 μm) microtomed sections of paraffin embedded specimens.
(ii) Formaldehyde-acetic acid-alcohol (FAA) was found to be satisfactory as a general purpose fixative for LM and low magnification SEM. However, for magnifications higher than X500, where subcellular details become the subject of investigation, glutaraldehyde (GA) was found to be preferable over FAA.
(iii) Certain artifacts appeared to be related to developmental stages of the peanut fruit.
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Keywords: Scanning electron microscopy, light microscopy, peanut fruit, Maturation, development
How to Cite:
Pattee, H. & Mohapatra, S. & Agnello, E.,
(1983) “Specimen Preparation Techniques for Scanning Electron Microscopy of Developing Peanut Pegs¹”,
Peanut Science 10(2),
01 Jan 1983