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In Vitro Culture to Eliminate Peanut Stripe Virus from Peanut Seed

Authors: R. D. V. J. Prasada Rao , G. Pio-Ribeiro , R. Pittman , D. V. R. Reddy , J. W. Demski

Abstract

When excised seed axes of peanut seed, which tested serological positive for peanut stripe virus (PStV), were grown in vitro, 37.5% of the developing plants were PStV-negative. When plants were regenerated from shoot-tips (1 cm long) taken from in vitro-grown, virus-infected plants, 29.4% of them were virus-negative. PStV-positive plants obtained from virus-infected seed were grown for 12 wk on Murashige and Skoog (MS) medium supplemented with 40 mg/L ribavirin (MSR) resulted in 78.6% which tested negative for PStV. All plants from seed which tested positive for PStV were 100% identified as negative if grown on MSR for 16 wk. Similarly all plants were PStV negative when obtained from 1 cm long infected shoot tips (from plants grown on MS or MSR medium) if subsequently grown for 12 wk on MSR medium.

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Keywords: Arachis hypogaea, excised seed axes, shoot tip, ribavirin, virus-free

How to Cite: Prasada Rao, R. , Pio-Ribeiro, G. , Pittman, R. , Reddy, D. & Demski, J. (1995) “In Vitro Culture to Eliminate Peanut Stripe Virus from Peanut Seed”, Peanut Science. 22(1). doi: https://doi.org/10.3146/pnut.22.1.0011