Field experiments were conducted to determine the effect of the dimethylamine salt of 2,4-DB at 0.45 kg ae/ha applied at various times during the growing season on runner peanut growth, development, and pod yield. The herbicide was applied once at 30, 45, 60, 90, or 120 d after planting. Additional treatments included 2,4-DB at 0.45 kg/ha applied twice beginning at 30, 60, or 90 d after planting or applied three times beginning at 30, 45, or 60 d after planting. The dates of application were selected to expose plants to the herbicide during all stages of development from prebloom through early pod development. Peanut yield and grade were not affected by 2,4-DB regardless of application timing or numbers of applications.