pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/pnut.22.1.0012 10.3146/i0095-3679-22-1-12 Articles Mechanical Inoculation Procedure to Screen for Resistance to Groundnut Rosette Virus in Peanut1 P. E. Olorunju 2, C. W. Kuhn 3, O. A. Ansa 2, S. M. Misari 2, and J. W. Demski 4, * Scientist, Inst. for Agric. Res., PMB 1044, Zaria, Nigeria Prof. Emeritus, Univ. of Georgia, Athens, GA Res. Leader, Dept. of Plant Pathology, Univ. of Georgia, Georgia Stn., Griffin, GA 30223

1Contribution from the Univ. of Georgia, College of Agric. and Environ. Sci. Supported in part by Peanut CRSP, USAID grant DAN-4048-G-00–0041–00.

 *Corresponding author. 1 1995 22 1 56 58 6 3 1995 American Peanut Research and Education Society 1995 American Peanut Research and Education Society Abstract

A mechanical inoculation procedure was developed for screening peanut plants for resistance to groundnut rosette virus (GRV) (green strain). A single inoculation resulted in 100% infection of plants of susceptible genotypes and about 2% of resistant plants which could be distinguished from susceptible ones on the basis of delayed time of first appearance of symptoms and disease severity. The procedure utilized highly infectious inoculum. The consistency and reliability of achieving infection should make the procedure particularly effective for inheritance of resistance studies and breeding programs.

 Key Words Virus transmission; , screening for resistance; , virus inoculation; , groundnut rosette virus