pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/pnut.22.1.0011 10.3146/i0095-3679-22-1-11 Articles In Vitro Culture to Eliminate Peanut Stripe Virus from Peanut Seed R. D. V. J. Prasada Rao 2, G. Pio-Ribeiro 3, R. Pittman 4, D. V. R. Reddy 5, and J. W. Demski 4, * Visiting Scientist, NBPGR campus, Rajendranagar, Hyderabad, India Visiting Scientist, Univ. of Pernambuco, Recife, Brazil Peanut Curator, USDA-ARS and Res. Leader, Dept. of Plant Pathology, Univ. of Georgia, Georgia Stn., Griffin, GA. 30223 Visiting Scientist, ICRISAT, Patancheru-P.O., India

1Contribution from the Univ. of Georgia, College of Agric. and Environ. Sci. Supported in part by Peanut CRSP, USAID grant DAN-4048-G-00–0041–00 and in part by State and Hatch funds allocated to the Univ. of Georgia.

 *Corresponding author. 1 1995 22 1 54 56 6 3 1995 American Peanut Research and Education Society 1995 American Peanut Research and Education Society Abstract

When excised seed axes of peanut seed, which tested serological positive for peanut stripe virus (PStV), were grown in vitro, 37.5% of the developing plants were PStV-negative. When plants were regenerated from shoot-tips (1 cm long) taken from in vitro-grown, virus-infected plants, 29.4% of them were virus-negative. PStV-positive plants obtained from virus-infected seed were grown for 12 wk on Murashige and Skoog (MS) medium supplemented with 40 mg/L ribavirin (MSR) resulted in 78.6% which tested negative for PStV. All plants from seed which tested positive for PStV were 100% identified as negative if grown on MSR for 16 wk. Similarly all plants were PStV negative when obtained from 1 cm long infected shoot tips (from plants grown on MS or MSR medium) if subsequently grown for 12 wk on MSR medium.

 Key Words Arachis hypogaea; , excised seed axes; , shoot tip; , ribavirin; , virus-free