pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/i0095-3679-18-1-1 Articles Identification of Peanut Stripe Virus Resistance in Wild Arachis Germplasm1,,2 R. D. V. J. Prasada Rao 3, A. S. Reddy 4, S. K. Chakrabarty 3, D. V. R. Reddy 4, *, V. R. Rao 4, and J. P. Moss 4 Plant Quarantine Regional Station of National Bureau of Plant Genetic Resources, Hyderabad 500 030 International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru P.O., Andhra Pradesh 502 324, India

1Submitted as Journal Article No. 1062 by the International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru P.O., Andhra Pradesh 502 324, India.

2Joint contribution from the Plant Quarantine Regional Station (PQRS) of National Bureau of Plant Genetic Resources (NBPGR) Hyderabad and International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru P.O., Andhra Pradesh 502 324, India.

 *Corresponding author. 1 1991 18 1 1 2 29 12 1990 American Peanut Research and Education Society 1991 American Peanut Research and Education Society Abstract

Fifty four wild Arachis germplasm accessions were evaluated for resistance to peanut stripe virus (PStV) by mechanical, aphid, and graft inoculations. Three weeks after inoculation, inoculated and subsequently produced leaflets of each entry were tested for PStV presence by enzyme-linked immunosorbent assay (ELISA). Peanut accessions in the section Arachis, A. cardenasii [ICG 11558 (PI 475998)] could not be infected by mechanical, aphid, and graft transmission tests, while A. chacoense [ICG 4983 (PI 276235)], A. cardenasii [ICG 11562 (PI 476012) and ICG 12168 (PI 476013)] and accessions of section Erectoides, A. sp. [ICG 11560 (PI 476004) and ICG 8215 (PI 468170)] and A. paraguariensis [ICG 8973 (PI 468176)], were infected by grafting, but not by aphid and mechanical inoculations.

 Key Words Arachis germplasm; , resistance; , peanut stripe virus; , direct antigen coating—enzyme-linked immunosorbent assay; , sap inoculation; , aphid inoculation; , graft inoculation