pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/i0095-3679-16-2-1 Articles Use of Monoclonal Antibodies in Detection and Serological Classification of Peanut Stripe Virus1 J. N. Culver , J. L. Sherwood *, and M. R. Sanborn 2 Former Graduate Research Assistant, Department of Plant Pathology, Associate Professor of Plant Pathology, and Associate Professor of Botany and Microbiology, Oklahoma State University, Stillwater, OK 74078

1Journal Article No. 5382, Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater, OK.

 *Corresponding Author. 7 1989 16 2 63 66 1 6 1989 American Peanut Research and Education Society 1989 American Peanut Research and Education Society Abstract

Monoclonal antibodies (MABs) to peanut stripe virus (PStV) were obtained by fusing spleen cells from mice immunized with PStV to a mouse myeloma cell line. Two IgG2a subclass MABs, each binding with different antigenic sites, and rabbit polyclonal antibodies (PABs) were compared for their reaction to PStV and other serologically related plant viruses. One MAB reacted to PStV but not to other serologically related viruses. The other MAB reaction of the MABs and PABs to PStV in peanut leaf and seed tissues were compared by enzyme-linked immunosorbent assay (ELISA), dot-immunobinding assay, and Western blotting. MABs were found to be equivalent to or better than PABs in detecting PStV by ELISA and dot-immunobinding assay, but one MAB was not suitable for Western blotting.

 Key Words Monoclonal antibodies; , peanut stripe virus; , serology