Standard grade samples (16) from each of 20 selected minilots were used to evaluate three methods for detecting minilots of farmers stock peanuts with unacceptable concentrations of aflatoxin. A visual, a minicolumn and a modified thin layer chromatography (TLC) method were used to compare analytical results, variation, and probability of acceptance for minilots having mean aflatoxin concentrations ranging from 8–255 ppb. Mean values obtained by each of the three methods increased linearly with mean aflatoxin concentrations of the minilots and variation for each method as determined by the variance and coefficient of variation (CV) was very large. The CV for all three methods decreased as aflatoxin concentration increased. Overall performances of the three methods were similar in accepting and rejecting these minilots on the basis of the 1.8 kg grade samples. The greatest difference in the three methods occurred at the zero acceptance level where the modified TLC, minicolumn and visual methods rejected 97, 98 and 88%, respectively, of the minilots with more than 60 ppb aflatoxin. At this acceptance level the TLC, minicolumn and visual methods also rejected 55, 50 and 30%, respectively, of the minilots with less than 30 ppb aflatoxin.