pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/i0095-3679-11-1-8 Articles In Vitro Differentiation of a Wild Peanut, Arachis villosulicarpa Hoehne1,,2 R. N. Pittman , B. B. Johnson , and D. J. Banks 3 Former Research Associate, CSRS, Langston University, Langston, Oklahoma, presently, Research Geneticist, USDA-ARS, Dept. of Agronomy, Oklahoma Agricultural Experiment Station; Associate Professor of Botany and Biochemistry, Oklahoma State University; and Research Geneticist, USDA-ARS, Dept. of Agronomy, Oklahoma Agricultural Experiment Station, Stillwater, OK 74078

1Cooperative investigations of Agricultural Research Service, U.S. Department of Agriculture and the Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74078. Journal article no. 4328, Oklahoma Agricultural Experiment Station.

2Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the Oklahoma Agricultural Experiment Station or the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.

 1 1984 11 1 24 27 21 1 1984 American Peanut Research and Education Society 1984 American Peanut Research and Education Society Abstract

Immature leaflets 3 to 5 mm in length from Arachis villosulicarpa Hoehne seedlings were cultured in vitro on a medium consisting of the major and minor salts of Murashige and Skoog (MS), Gamborg's B5 vitamins, 20 g/L sucrose, 0.8% Difco agar, 4.44 μM N-6 benzyladenine (BA) and 5.37 μM napthaleneacetic acid (NAA). Hard callus with many shoot primordia formed on all leaflets within 4 to 6 weeks. Calli with primordia were further cultured for plant formation on 150 other media (MS major salts at 1/2 or 1/4 strength, full strength minor salts and vitamins, sucrose at 5 or 10 g/L, BA and NAA at 0 to 8 μM). Reduced concentrations of major salts with a high ratio of BA to NAA enhanced the formation of shoot primordia. Roots differentiated on media with 1/4 strength major salts, no BA, and 6 to 8 μM NAA and 5 g/L sucrose. Shoots differentiated from the branch points of roots after 2 to 4 months of culture. Plants were obtained via shoot primordia from callus and from the adventitious shoots from roots.

 Key Words Groundnut; , Morphogenesis; , Organogenesis; , SEM; , Tissue culture