pnut pnut Peanut Science 0095-3679 0095-3679 American Peanut Research and Education Society 10.3146/i0095-3679-10-1-7 Articles In Vitro Culture of Immature Peanut (Arachis spp.) Leaves: Morphogenesis and Plantlet Regeneration1,2 R. N. Pittman , D. J. Banks , J. S. Kirby , E. D. Mitchell , and P. E. Richardson 3 Former Graduate Research Assistant (Presently, Research Associate, Department of Agronomy, Oklahoma State University); Research Geneticist, USDA, ARS; Associate Professor of Agronomy, Professor of Biochemistry, and Professor of Botany, Departments of Agronomy, Biochemistry, and Botany, respectively, Oklahoma State University, Stillwater, OK 74078

1Cooperative investigations of Agricultural Research Service, USDA, and Oklahoma State University. Journal Article No. 4212, Oklahoma Agricultural Experiment Station, Oklahoma State University, Stillwater, OK 74078. Part of a dissertation submitted by the senior author for the Ph. D. degree.

2Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by Oklahoma State University or the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable.

 1 1983 10 1 21 25 23 2 1983 American Peanut Research and Education Society 1983 American Peanut Research and Education Society Abstract

Green immature leaflets (2-5 mm in length from shoots of germinated seeds or greenhouse grown plants) from species representing seven taxonomic sections of the genus Arachis (Ambinervosae, Arachis, Caulorhizae, Erectoides, Extranervosae, Rhizomatosae, and Triseminalae), were cultured aseptically, in vitro, on a medium composed of Murashige and Skoog salts, Gamborg's B5 vitamins, 0.8% Difco agar, and supplemented with 1 mg/L each of naphthaleneacetic acid and N-6 benzyladenine. Histological examination of the cultures revealed that the meristematic areas originated from epidermal cells. Embryoids and meristematic shoots developed after lysis of the surrounding cells. All species of Arachis tested produced callus. Genotypic differences for the production of callus, shoots, and roots were observed with cultivated peanuts. Organogenesis occurred in the leaflet cultures, and plants were recovered from sections Arachis and Extranervosae of the genus Arachis.

 Key Words Leaf explants; , organogenesis; , tissue culture