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	<front>
		<journal-meta>
			<journal-id journal-id-type="publisher-id">pnut</journal-id>
			<journal-title-group>
				<journal-title>Peanut Science</journal-title>
			</journal-title-group>
			<issn pub-type="active">0095-3679</issn>
			<issn pub-type="ppub">0095-3679</issn>
			<publisher>
				<publisher-name>American Peanut Research and Education Society</publisher-name>
			</publisher>
		</journal-meta>
		<article-meta>
			<article-id pub-id-type="doi">10.3146/PS17-19.1</article-id>
			<article-id pub-id-type="sici">pnut-45-01-05</article-id>
			<article-categories>
				<subj-group subj-group-type="heading">
					<subject>ARTICLES</subject>
				</subj-group>
			</article-categories>
			<title-group>
				<article-title>Weed Control and Peanut (<italic toggle="yes">Arachis hypogaea</italic> L.) Response to Acetochlor Alone and in Combination with Various Herbicides</article-title>
				<alt-title alt-title-type="runhead">Peanut Response to Acetochlor</alt-title>
			</title-group>
			<contrib-group>
				<contrib contrib-type="author">
					<string-name name-style="western">
						<given-names>Sushila</given-names>
						<surname>Chaudhari</surname></string-name>
					<xref rid="n101" ref-type="fn"><sup>1</sup></xref><x xml:space="preserve">, </x>
				</contrib>
				<contrib contrib-type="author">
					<string-name name-style="western">
						<given-names>David L.</given-names>
						<surname>Jordan</surname></string-name>
					<xref rid="n101" ref-type="fn"><sup>1</sup></xref>
					<xref rid="cor1" ref-type="corresp"><sup>*</sup></xref><x xml:space="preserve">, </x>
				</contrib>
				<contrib contrib-type="author">
					<string-name name-style="western">
						<given-names>Timothy L.</given-names>
						<surname>Grey</surname></string-name>
					<xref rid="n102" ref-type="fn"><sup>2</sup></xref><x xml:space="preserve">, </x>
				</contrib>
				<contrib contrib-type="author">
					<string-name name-style="western">
						<given-names>Eric P.</given-names>
						<surname>Prostko</surname></string-name>
					<xref rid="n102" ref-type="fn"><sup>2</sup></xref><x xml:space="preserve">, and </x>
				</contrib>
				<contrib contrib-type="author">
					<string-name name-style="western">
						<given-names>Katherine M.</given-names>
						<surname>Jennings</surname></string-name>
					<xref rid="n103" ref-type="fn"><sup>3</sup></xref>
				</contrib>
			</contrib-group>
			
			<pub-date pub-type="ppub">
				<month>1</month>
				<year>2018</year>
			</pub-date>
			<volume>45</volume>
			<issue>1</issue>
			<fpage>45</fpage>
			<lpage>55</lpage>
			<permissions><copyright-statement></copyright-statement>
				<copyright-year>2018</copyright-year>
			</permissions>
			<related-article related-article-type="pdf" xlink:href="PS17-19.1.pdf"></related-article>
			<abstract>
				<title>ABSTRACT</title>
				<p>Acetochlor, a chloroacetamide herbicide, is now registered for preplant (PPI), preemergence (PRE), and postemergence (POST) application in peanut. Field research was conducted during 2011 and 2012 in Georgia and North Carolina to determine peanut response and weed control by acetochlor compared with <italic toggle="yes">S</italic>-metolachlor alone and in programs with other herbicides. In weed-free experiments, peanut tolerance to acetochlor (1.26 and 2.52 kg ai/ha) and <italic toggle="yes">S</italic>-metolachlor (1.42 kg ai/ha) were evaluated when applied PPI, PRE, early postemergence (EPOST), or POST. Peanut tolerance to acetochlor was similar to <italic toggle="yes">S</italic>-metolachlor with no negative impact of either herbicide on peanut yield compared with non-treated peanut in absence of weed interference. When applied PRE, acetochlor controlled Palmer amaranth, pitted morningglory, sicklepod, and Texas millet similarly to <italic toggle="yes">S</italic>-metolachlor while control of broadleaf signalgrass was greater with <italic toggle="yes">S</italic>-metolachlor. Weed control programs containing EPOST and/or POST applications of herbicides following PRE herbicides provided the best overall weed control but did not affect yellow nutsedge control regardless of whether acetochlor or <italic toggle="yes">S</italic>-metolachlor were applied. Herbicide programs including PRE, EPOST, and POST herbicides most often resulted in the greatest yields. There was no difference in peanut yield regardless of the presence of acetochlor or <italic toggle="yes">S</italic>-metolachlor in a comprehensive herbicide program.</p>
			</abstract>
			<kwd-group>
				<title>Key Words</title>
				<kwd>Crop injury</kwd><x xml:space="preserve">, </x>
				<kwd>herbicide mixture</kwd><x xml:space="preserve">, </x>
				<kwd>peanut tolerance</kwd><x xml:space="preserve">.</x>
			</kwd-group>
		</article-meta>
	</front>
	<body>
		<sec id="s1">
			<title>Introduction</title>
			<p>Peanut is a valuable commodity in the United States with approximately 634,800 ha harvested with an estimated value of $1.19 billion (<xref ref-type="bibr" rid="i0095-3679-45-1-45-USDA1">USDA 2016a</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-USDA2">USDA 2016b</xref>). Weeds compete with peanut for sunlight, moisture, and nutrients throughout the growing season (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>), and negatively affect yield, quality, and economic value (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Everman1">Everman <italic toggle="yes">et al</italic>., 2008</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Walker1">Walker <italic toggle="yes">et al</italic>., 1989</xref>). Season-long interference from combinations of broadleaf and grass weeds can reduce peanut yield by 60 to 80% and can decrease harvest efficiency in some instances (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Everman1">Everman <italic toggle="yes">et al</italic>., 2008</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>). In studies investigating the effect of season-long interference from individual weed species, common ragweed (<italic toggle="yes">Ambrosia artemisiifolia</italic> L.) (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis1">Clewis <italic toggle="yes">et al</italic>., 2001</xref>) and Palmer amaranth [<italic toggle="yes">Amaranthus palmeri</italic> (S.) Wats] (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Burke1">Burke <italic toggle="yes">et al</italic>., 2007</xref>) at a density of 1 plant/m of row, resulted in peanut yield losses of 40 and 28%, respectively. Competition from Texas millet [<italic toggle="yes">Urochloa texana</italic> (Buckl.)] and Florida beggarweed [<italic toggle="yes">Desmodium tortuosum</italic> (Sw.) DC.] can also severely reduce peanut yield (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Buchanan1">Buchanan et al., 1976</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Cardina1">Cardina and Brecke, 1991</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut3">Wilcut, <italic toggle="yes">et al</italic>., 1987</xref>). The prostrate growth habit of peanut causes peanut to be vulnerable to interference from weeds throughout the season and requires effective season-long management to protect yield and promote efficient digging and vine inversion (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Walker1">Walker <italic toggle="yes">et al</italic>., 1989</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>).</p>
			<p><italic toggle="yes">S</italic>-metolachlor is a chloroacetamide herbicide, labeled for either pre-plant incorporated (PPI), preemergence (PRE), postemergence (POST), or lay-by application in peanut (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Anonymous1">Anonymous, 2017a</xref>). Peanut stunting and delayed emergence with <italic toggle="yes">S</italic>-metolachlor has been noted in previous studies (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Cardina2">Cardina and Swann, 1988</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>., 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray, 2012</xref>). <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray (2012)</xref> reported peanut stunting from 0 to 15% with <italic toggle="yes">S</italic>-metolachlor alone and in combination with paraquat, and stunting increased as application timing was delayed to 28 d compared to 7 d after peanut cracking. Peanut injury by <italic toggle="yes">S</italic>-metolachlor and the racemic form of metolachlor is dependent on combinations of factors, including herbicide rate, moisture conditions at planting, soil temperature, and rainfall (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Cardina2">Cardina and Swann, 1988</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar3">Grichar <italic toggle="yes">et al</italic>., 2004</xref>). <xref ref-type="bibr" rid="i0095-3679-45-1-45-Cardina2">Cardina and Swann (1988)</xref> reported that peanut growth suppression was related to higher metolachlor rates followed by irrigation after planting. Although some level of injury was reported in peanut from metolachlor, negative effects were not observed on peanut market grades and yield unless rates higher than those recommended by the manufacturer were applied. <italic toggle="yes">S</italic>-metolachlor provides control of a number of troublesome annual broadleaf weeds and yellow nutsedge when applied alone or in combination with sulfentrazone, diclosulam, or flumioxazin (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>., 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar5">Grichar <italic toggle="yes">et al</italic>., 2008</xref>).</p>
			<p>Acetochlor is registered for use in field corn (<italic toggle="yes">Zea mays</italic> L.), cotton <italic toggle="yes">(Gossypium hirsutum</italic> L.), sorghum [<italic toggle="yes">Sorghum bicolor</italic> (L.) Moench.], and soybean [<italic toggle="yes">Glycine max</italic> (L.) Merr.] to control annual grasses and small-seeded broadleaf weeds (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Anonymous2">Anonymous, 2017b</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Cahoon1">Cahoon <italic toggle="yes">et al</italic>. 2015</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Geier1">Geier <italic toggle="yes">et al</italic>., 2009</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Steckel1">Steckel <italic toggle="yes">et al</italic>., 2002</xref>). Acetochlor inhibits geranylgeranyl pyrophosphate cyclization enzymes, which are part of the gibberellin biosynthetic pathway and controls weeds by inhibiting growth of seedling shoots (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Shaner1">Shaner, 2014</xref>). A microencapsulated (ME) formulation of acetochlor recently received registration for PPI, PRE, and POST application in peanut (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Anonymous2">Anonymous 2017b</xref>). In addition to improving crop safety, this formulation of acetochlor extends herbicide persistence in the soil compared with the emulsifiable concentrate formulation (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Parker1">Parker <italic toggle="yes">et al</italic>., 2005</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Scher1">Scher <italic toggle="yes">et al</italic>., 1998</xref>). <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar4">Grichar <italic toggle="yes">et al</italic>. (2015)</xref> reported no negative effect of acetochlor ME application timing and rate on peanut grade and yield in runner and Spanish market type cultivars. <xref ref-type="bibr" rid="i0095-3679-45-1-45-Cahoon1">Cahoon <italic toggle="yes">et al</italic>. (2015)</xref> documented that acetochlor ME applied PRE caused less than 8% early season injury to cotton and at least 90% control of Palmer amaranth. Early season injury caused by acetochlor was transient and did not negatively impact cotton yield.</p>
			<p>One effective approach to manage weeds with long periods of emergence is to use sequential applications of PRE and POST herbicides. <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar5">Grichar <italic toggle="yes">et al</italic>. (2008)</xref> reported &gt; 80% yellow nutsedge (<italic toggle="yes">Cyperus esculentus</italic> L.) control at five of six locations in peanut with sequential application of diclosulam PRE followed by <italic toggle="yes">S</italic>-metolachlor applied POST. Control of Palmer amaranth, common lambsquarters (<italic toggle="yes">Chenopodium album</italic> L.), and annual morningglory (<italic toggle="yes"><named-content content-type="genus-species">Ipomoea</italic></italic> spp.) in peanut was improved when a POST application of imazapic plus 2,4-DB followed PRE <italic toggle="yes">S</italic>-metolachlor alone or in combination with diclosulam, flumioxazin, or sulfentrazone, compared with PRE- or POST-only treatments (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>. 2007</xref>). <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar4">Grichar <italic toggle="yes">et al</italic>. (2015)</xref> reported that the addition of pendimethalin and lactofen to acetochlor, flumioxazin, or <italic toggle="yes">S</italic>-metolachlor programs improved weed control in peanut.</p>
			<p>Peanut producers typically include PPI, PRE, EPOST, and POST herbicide applications in their weed management programs for effective season-long control of nutsedge, annual grass, and broadleaf weeds. Dinitroaniline herbicides, such as ethalfluralin, pendimethalin, or trifluralin, are applied PPI to control many annual grass and small-seeded broadleaf weeds (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray, 2012</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>). Diclosulam, <italic toggle="yes">S</italic>-metolachlor, flumioxazin, and sulfentrazone are registered PRE (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>., 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Jordan1">Jordan, 2016</xref>). In addition to these herbicides, acifluorfen, bentazon, clethodim, imazapic, imazethapyr, paraquat, sethoxydim, and 2,4-DB are used in peanut for POST weed control (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Grey2">Grey <italic toggle="yes">et al</italic>., 2003</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>). Herbicide resistance in weeds has increased concerns about proper herbicide stewardship (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Wise1">Wise <italic toggle="yes">et al</italic>., 2009</xref>). The registration of acetochlor will increase herbicide options for weed control in peanut. Considering the need for soil-applied residual herbicides to manage weeds and the limited published information on acetochlor tolerance to peanut, field research was conducted in Georgia and North Carolina to define utility of acetochlor compared with <italic toggle="yes">S</italic>-metolachlor. The specific objectives of this research were: 1) to determine peanut response to the ME formulation of acetochlor with <italic toggle="yes">S</italic>-metolachlor when applied at different rates and timings under weed-free conditions and 2) to compare weed control with the ME formulation of acetochlor to control by <italic toggle="yes">S</italic>-metolachlor alone or in various herbicide programs which includes combinations with acifluorfen, bentazon, flumioxazin, imazapic, lactofen, paraquat, and pendimethalin.</p>
		</sec>
		<sec id="s2">
			<title>Material and Methods</title>
			<p>Field experiments were conducted in Georgia at the Ponder Research Station near Ty Ty, the Attapulgus Research and Education Center near Attapulgus, the Southwest Georgia Research and Education Center near Plains, and in North Carolina at the Peanut Belt Research Station located near Lewiston-Woodville, and the Upper Coastal Plain Research Station near Rocky Mount during 2011 and 2012. Soil was a Tifton loamy sand soil (fine-loamy, kaolinitic, thermic Plinthic Kandiudult) with 1.07 to 1.39% organic matter (OM) and pH 6.0 at Ty Ty, an Orangeburg loamy sand soil (Fine-loamy, kaolinitic, thermic Typic Kandiudult) with 1.07 to 1.19% OM and pH 6.0 at Attapulgus, and a Greenville sandy clay loam soil (Fine, kaolinitic, thermic Rhodic Kandiudult) with 1.0% OM and pH 5.9 to 6.1 at Plains. Soil at Lewiston-Woodville was a Norfolk sandy loam soil (fine-loamy, siliceous, thermic, Aquic Paleudult) with 0.8 to 1.2% OM and pH 5.9. Goldsboro loamy sand soil (fine loamy, mixed, semiactive, thermic, Typic Hapludult) with 1.4% organic matter and pH 6.1 was at Rocky Mount. Soils at these locations are representative of the southeastern United States peanut production region. Experiments were classified as specific combinations of year and/or location within each state. Experiments were conducted in conventionally-prepared, raised seedbeds. Plot size was 4 rows spaced 96-cm apart by 9 m in North Carolina and 2 rows spaced 91-cm apart by 7.6 m in Georgia. Peanut was planted at a depth of 4 to 5 cm at all locations at a rate designed to achieve final in-row populations of 4 and 6 plants/m in North Carolina and Georgia, respectively. Production, irrigation, and pest management practices other than specific treatments were standard for peanut production in Georgia and North Carolina to optimize peanut growth and development (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Anonymous3">Anonymous, 2017c</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Jordan2">Jordan <italic toggle="yes">et al</italic>., 2016</xref>).</p>
			<sec id="s2a">
				<title>Peanut Tolerance</title>
				<p>Peanut cultivars (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Branch1">Branch 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Mozingo1">Mozingo <italic toggle="yes">et al</italic>. 2006</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Isleib2">Isleib <italic toggle="yes">et al</italic>., 2006</xref>, <xref ref-type="bibr" rid="i0095-3679-45-1-45-Isleib1">2011</xref>), planting dates, and dates of herbicide application are presented in <xref ref-type="table" rid="i0095-3679-45-1-45-t01">Table 1</xref>. Treatments consisted of a factorial arrangement of 3 levels of herbicide and 4 levels of application timings. Herbicide levels included acetochlor (Warrant herbicide, Monsanto Co., St, Louis, MO) at 1.26 and 2.52 kg/ha and <italic toggle="yes">S</italic>-metolachlor (Dual Magnum herbicide, Syngenta Crop Protection, Greensboro, NC) at 1.42 kg/ha. These herbicides were applied PPI, PRE, EPOST, or POST. A non-treated control was also included. Herbicides were applied in water using a CO<sub>2</sub>-pressurized backpack sprayer calibrated to deliver 140 L/ha using 11002 flat fan (TeeJet Technologies, Wheaton, IL) nozzles at 150 kPa at Plains, 11002DG flat fan nozzles at 260 kPa at Ty Ty, and 8002 flat fan nozzles at 200 kPa at Lewiston-Woodville and Rocky Mount. PPI herbicides were incorporated immediately after application with a power-driven rotary tiller (Plains and Ty Ty) or with 2 passes with a field cultivator in opposite directions at Lewiston-Woodville and Rocky Mount to a depth of approximately 5 cm. Herbicides were applied PRE within 24 h after peanut planting. In Georgia, 3 cm of overhead sprinkler irrigation were applied within 1 wk after application of PRE herbicides. Peanut was not irrigated after planting in North Carolina. EPOST applications were made approximately 14 to 24 d after planting when peanut was approximately at V3 to V4 stage of growth (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Boote1">Boote, 1982</xref>) while POST applications were made 25 to 38 d after peanut planting when peanut was at R1 stage of growth (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Boote1">Boote, 1982</xref>).</p>
				<table-wrap id="i0095-3679-45-1-45-t01" position="float" content-type="2col" orientation="portrait">
					<label>Table 1.</label>
					<caption>
						<p>Planting and herbicide application dates evaluating peanut tolerance under weed-free conditions in Georgia and North Carolina, 2011-2012<sup>a</sup>.</p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t01.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<p>All plots were kept weed-free combining hand removal with herbicides applied PRE and POST. Diclosulam (Strongarm herbicide, Dow AgroSciences, Indianapolis, IN) at 0.026 kg ai/ha, flumioxazin (Valor SX, Valent USA Corp., Walnut Creek, CA) at 0.11 kg ai/ha, or pendimethalin (Prowl H<sub>2</sub>O, BASF Corp., Research Triangle Park, NC) at 1.13 kg ai/ha were applied PRE. Clethodim (Select herbicide, Valent USA Corp., Walnut Creek, CA) at 0.13 kg ai/ha or imazapic (Cadre herbicide, BASF Corp., Research Triangle Park, NC) at 0.70 kg ai /ha with crop oil concentrate applied POST to control annual grasses and broadleaf weeds at Plains and Ty Ty. Pendimethalin at 1.1 kg ai/ha was applied PPI and clethodim at 130 g/ha, and 2,4-DB (Butyrac 200, Winfield Solutions, LLC, St. Paul, MN) at 0.28 kg ai/ha were applied POST for weed control at Lewiston-Woodville and Rocky Mount.</p>
				<p>Experiments were conducted in a randomized complete block design with four replications. Visual estimates of peanut injury were determined 2-3 and 6-7 weeks after each application timing (WAT) using a scale of 0 to 100 where 0 = no injury and 100 = plant death. Foliar chlorosis, necrosis, leaf defoliation, and plant stunting were considered when making the visible estimates. Peanut pods were dug and vines inverted based on pod mesocarp color to obtain optimum yield (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Williams1">Williams and Drexler, 1981</xref>). Pod yield was determined 4 to 7 d after digging with final yield adjusted to 8% moisture content.</p>
			</sec>
			<sec id="s2b">
				<title>Weed Control</title>
				<p>Field experiments were conducted in Georgia at Plains and Attapulgus and in North Carolina at Lewiston-Woodville and Rocky Mount during 2011 and 2012. Peanut cultivars, planting dates, and herbicide application dates are presented in <xref ref-type="table" rid="i0095-3679-45-1-45-t02">Table 2</xref>. Treatments consisted of a factorial arrangement of two base herbicides (<italic toggle="yes">S</italic>-metolachlor and acetochlor ME) and five herbicide programs with base herbicide applied at different timings (<xref ref-type="table" rid="i0095-3679-45-1-45-t03">Table 3</xref>). A non-treated control was also included. Herbicides were applied using equipment and procedures described in the study evaluating peanut response under weed-free conditions. Preemergence herbicides were applied within 24 h after peanut planting while EPOST and POST herbicides were applied approximately 15 to 22 and 25 to 38 d after peanut was planted, respectively.</p>
				<table-wrap id="i0095-3679-45-1-45-t02" position="float" content-type="2col" orientation="portrait">
					<label>Table 2.</label>
					<caption>
						<p>Planting and herbicide application dates for peanut weed control research in Georgia and North Carolina, 2011-2012.<sup>a</sup></p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t02.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<table-wrap id="i0095-3679-45-1-45-t03" position="float" content-type="2col" orientation="portrait">
					<label>Table 3.</label>
					<caption>
						<p>Herbicide timing and rate of application in experiments determining weed control with PRE, EPOST, and POST herbicides. <sup>a</sup></p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t03.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<p>Weed control was estimated visually using a scale of 0 (no weed control) to 100 (complete weed control) 14 to 69 d after peanut were planted depending on location. Peanut injury (chlorosis/stunting) was also recorded at 3 to 8 d after POST application at Plains and Lewiston-Woodville both years and Rocky Mount in 2012 while at Attapulgus both years and Rocky Mount 2011 at 8 d after EPOST herbicide application using the scale described previously. Peanut yield was determined as described previously.</p>
			</sec>
			<sec id="s2c">
				<title>Data Analysis</title>
				<p>Data for peanut injury were transformed to the arcsine square root before analysis; however, non-transformed means are presented for clarity. Data were subjected to ANOVA using SAS PROC MIXED considering the factorial treatment arrangement (SAS Institute Inc., Cary, NC). Treatment means were separated by Fisher's Protected LSD test at p &alpha; 0.05. The non-treated check was not included in the weed control or peanut injury analysis but was included in peanut yield analysis. Peanut population, visible injury, and yield data from both peanut tolerance and weed control studies were analyzed separately for Georgia and North Carolina because of variation in cultivar selection, and timing for data collection and weed spectrum. However, percent weed control data were analyzed across states and data were combined if the interaction of experiment by base herbicide by herbicide program was not significant.</p>
			</sec>
		</sec>
		<sec id="s3">
			<title>Results and Discussion</title>
			<sec id="s3a">
				<title>Peanut Tolerance</title>
				<p>The interaction of experiment by herbicide treatment by application timing was not significant for peanut population and pod yield in Georgia (P=0.31 and 0.38, respectively) and North Carolina (P=0.76 and 0.23, respectively); therefore, data were combined over experiments for both states. However, this three-way interaction was significant for peanut injury at 2 WAT for Georgia (P = 0.0001) and 2-3 WAT for North Carolina (P = 0.009); therefore, data are presented by experiment. Further analysis indicated that the interaction of herbicide by timing of application was not significant for peanut injury, peanut population, and pod yield. Therefore, data for the main effects of herbicide and timing of application are presented.</p>
				<p>Peanut injury 2 WAT during 2011 and 2012 at Lewiston-Woodville and 2012 at Rocky Mount was not affected by herbicide treatment (<xref ref-type="table" rid="i0095-3679-45-1-45-t04">Table 4</xref>). In contrast, at Rocky Mount during 2011, <italic toggle="yes">S</italic>-metolachlor injured peanut less than acetochlor at 1.26 kg/ha. However, similar injury was observed for acetochlor and <italic toggle="yes">S</italic>-metolachlor when acetolchlor was applied at 2.52 kg/ha. Injury did not exceed 6% regardless of herbicide or rate of acetochlor. Significant but minor differences in injury (6% or less) were observed when comparing method and timing of application at these locations (<xref ref-type="table" rid="i0095-3679-45-1-45-t04">Table 4</xref>). Peanut injury in Georgia did not exceed 5% when comparing herbicides pooled over application timing (<xref ref-type="table" rid="i0095-3679-45-1-45-t05">Table 5</xref>). In 1 of 4 experiments injury by <italic toggle="yes">S</italic>-metolachlor exceeded that of the lower rate of acetochlor. A consistent trend was not observed when comparing application timings, although injury was 8% or less. Injury was transient, and by 4 to 5 wk at Lewiston-Woodville and Rocky Mount and 6 to 7 wk after treatments at Ty Ty and Plains, injury was 1% or less (data not shown).</p>
				<table-wrap id="i0095-3679-45-1-45-t04" position="float" content-type="2col" orientation="portrait">
					<label>Table 4.</label>
					<caption>
						<p>Peanut injury from acetochlor and <italic toggle="yes">S</italic>-metolachlor at different application timings under weed-free conditions in North Carolina<sup>a,b</sup>.</p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t04.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<table-wrap id="i0095-3679-45-1-45-t05" position="float" content-type="2col" orientation="portrait">
					<label>Table 5.</label>
					<caption>
						<p>Peanut injury from acetochlor and <italic toggle="yes">S</italic>-metolachlor at different application timings under weed-free conditions in Georgia (Study 1)<sup>a,b</sup>.</p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t05.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<p>No differences were noted with herbicide treatment or application timing for peanut population or yield in either state or year (<xref ref-type="table" rid="i0095-3679-45-1-45-t06">Table 6</xref>). Visible injury from metolachlor does not always result in lower peanut yield compared with yield of non-treated peanut under weed-free conditions when applied at rates recommended in by the manufacturer (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Cardina2">Cardina and Swann, 1988</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>., 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray, 2012</xref>). In a study similar to the one reported in this article, <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar4">Grichar <italic toggle="yes">et al</italic>. (2015)</xref> observed in Texas that peanut yield and market grade characteristics were not affected by acetochlor rate or application timing (PPI, PRE, EPOST, POST). The indeterminate growth habit of peanut often enables peanut to recover from early season stress including herbicide injury and yield is often not adversely affected (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Carley1">Carley <italic toggle="yes">et al</italic>., 2009</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray, 2012</xref>).</p>
				<table-wrap id="i0095-3679-45-1-45-t06" position="float" content-type="2col" orientation="portrait">
					<label>Table 6.</label>
					<caption>
						<p>Peanut population and yield response to acetochlor and <italic toggle="yes">S</italic>-metolachlor at different application timings under weed-free conditions in Georgia and North Carolina<sup>a,b</sup>.</p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t06.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
			</sec>
			<sec id="s3b">
				<title>Weed Control</title>
				<p>The interaction for experiment by base herbicide by herbicide program was significant for Palmer amaranth control (P=0.001); therefore, data are presented by experiment. In contrast, lack of a significant experiment by base herbicide by herbicide program interaction allowed the combining of data over experiments for broadleaf signalgrass [<italic toggle="yes">Urochloa platyphylla</italic> (Nash) R.D. Webster] control (P=0.39), pitted morningglory (<italic toggle="yes">Ipomoea lacunosa</italic> L.) control (P=0.08), sicklepod [<italic toggle="yes">Senna obtusifolia</italic> (L.) H.S. Irwin &#x26; Barneby] control (P=0.26), and Texas millet control (P=0.39). The interaction of base herbicide by herbicide program was not significant for control of Palmer amaranth, broadleaf signalgrass, pitted morningglory, and sicklepod; therefore, data for the main effects are presented (<xref ref-type="table" rid="i0095-3679-45-1-45-t07">Tables 7</xref> and <xref ref-type="table" rid="i0095-3679-45-1-45-t08">8</xref>). In contrast, the interaction of base herbicide by herbicide program was significant for Texas millet control (<xref ref-type="table" rid="i0095-3679-45-1-45-t09">Table 9</xref>).</p>
				<table-wrap id="i0095-3679-45-1-45-t07" position="float" content-type="2col" orientation="portrait">
					<label>Table 7.</label>
					<caption>
						<p>Palmer amaranth control in peanut with herbicide programs containing acetochlor and <italic toggle="yes">S</italic>-metolachlor.<sup>a</sup></p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t07.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<table-wrap id="i0095-3679-45-1-45-t08" position="float" content-type="2col" orientation="portrait">
					<label>Table 8.</label>
					<caption>
						<p>Broadleaf signalgrass, pitted morningglory, and sicklepod control in peanut with herbicide programs containing acetochlor and <italic toggle="yes">S</italic>-metolachlor.<sup>a</sup></p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t08.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<table-wrap id="i0095-3679-45-1-45-t09" position="float" content-type="2col" orientation="portrait">
					<label>Table 9.</label>
					<caption>
						<p>Texas millet control in peanut with herbicide programs containing acetochlor and <italic toggle="yes">S</italic>-metolachlor.<sup>a</sup></p>
					</caption>
					<graphic xlink:href="i0095-3679-45-1-45-t09.png" position="float" orientation="portrait"></graphic>
				</table-wrap>
				<sec id="s3b1">
					<title>Palmer amaranth</title>
					<p>There was no difference between acetochlor and <italic toggle="yes">S</italic>-metolachlor for Palmer amaranth control except at Plains in 2011, where greater control was obtained from acetochlor compared to <italic toggle="yes">S</italic>-metolachlor (<xref ref-type="table" rid="i0095-3679-45-1-45-t07">Table 7</xref>). At Plains in 2011 and 2012 and Rocky Mount in 2011, herbicide programs containing only base herbicides provided less Palmer amaranth control compared to other herbicide programs. However, at Rocky Mount in 2012, the herbicide program including pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus acetochlor or <italic toggle="yes">S</italic>-metolachlor POST controlled Palmer amaranth 94% while all other herbicide programs controlled this weed less than 88% (<xref ref-type="table" rid="i0095-3679-45-1-45-t06">Table 6</xref>). <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar1">Grichar et al. (2005)</xref> reported greater control of Palmer amaranth with POST herbicides following PPI application of <italic toggle="yes">S</italic>-metolachlor was at least 84% compared to only 69% by <italic toggle="yes">S</italic>-metolachlor. Addition of EPOST or POST herbicides with PPI or PRE herbicides often are beneficial in controlling Palmer amaranth that escapes weed control programs early in the year or when this weed emerges later in the season. <xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>. (2007)</xref> reported that a EPOST application of paraquat plus bentazon fb the prepackage mixture of acifluorfen plus bentazon POST plus 2,4-DB or imazapic plus 2,4-DB POST controlled Palmer amaranth 93% and 97%.</p>
				</sec>
				<sec id="s3b2">
					<title>Broadleaf signalgrass</title>
					<p><italic toggle="yes">S</italic>-metolachlor controlled 86% broadleaf signalgrass which was greater than control by acetochlor (<xref ref-type="table" rid="i0095-3679-45-1-45-t08">Table 8</xref>). Broadleaf signalgrass can rapidly emerge under warm conditions and throughout the growing season in high numbers (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Burke2">Burke <italic toggle="yes">et al</italic>., 2003</xref>). Therefore, multiple herbicide applications are generally needed for season-long control. The herbicide program including pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus acetochlor or <italic toggle="yes">S</italic>-metolachlor POST controlled broadleaf signalgrass 91%. <xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>. (2007)</xref> reported that imazapic plus 2,4-DB POST followed by PRE and EPOST herbicide application controlled broadleaf signalgrass at least 96%.</p>
				</sec>
				<sec id="s3b3">
					<title>Pitted morningglory</title>
					<p>There was no difference between acetochlor and <italic toggle="yes">S</italic>-metolachlor for pitted morningglory control (<xref ref-type="table" rid="i0095-3679-45-1-45-t08">Table 8</xref>). The most effective herbicide program for pitted morningglory was pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST which provided 90% control.</p>
				</sec>
				<sec id="s3b4">
					<title>Sicklepod</title>
					<p>There was no difference between acetochlor and <italic toggle="yes">S</italic>-metolachlor for sicklepod control (<xref ref-type="table" rid="i0095-3679-45-1-45-t08">Table 8</xref>). Herbicide program including pendimethalin plus flumioxazin PRE fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST and pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST controlled sicklepod at least 93%, while all other herbicide programs provided ± 41% control. Previous studies have documented effective control of sicklepod by imazapic POST or flumioxazin PRE followed by paraquat plus bentazon EPOST (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Grey1">Grey and Wehtje, 2005</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Webster1">Webster <italic toggle="yes">et al</italic>., 1997</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut4">Wilcut <italic toggle="yes">et al</italic>., 1996</xref>).</p>
				</sec>
				<sec id="s3b5">
					<title>Texas millet</title>
					<p>Acetochlor and <italic toggle="yes">S</italic>-metolachlor alone provided 53% control of Texas millet (<xref ref-type="table" rid="i0095-3679-45-1-45-t09">Table 9</xref>). Previous research (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut2">Wilcut <italic toggle="yes">et al</italic>., 1995</xref>) indicated that the racemic form of metolachlor provides little or no Texas millet control; however, the dinitroaniline herbicides provide excellent control of annual grasses (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut1">Wilcut <italic toggle="yes">et al</italic>., 1994</xref>). Imazapic controls Texas millet and broadleaf signalgrass (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar1">Grichar <italic toggle="yes">et al</italic>., 2005</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Wilcut2">Wilcut <italic toggle="yes">et al</italic>., 1995</xref>). Herbicide programs including pendimethalin plus flumioxazin PRE fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST and pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus acetochlor or <italic toggle="yes">S</italic>-metolachlor POST controlled Texas millet at least 87%, while all other herbicide programs provided ± 77% control. <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar1">Grichar <italic toggle="yes">et al</italic>. (2005)</xref> reported that <italic toggle="yes">S</italic>-metolachlor PRE in combination with imazapic POST provided 94% control of Texas millet which was better than S-metolachlor alone.</p>
				</sec>
				<sec id="s3b6">
					<title>Peanut injury and yield</title>
					<p>The interaction of experiment by base herbicide by herbicide program was not significant for peanut yield for North Carolina (P=0.90); therefore data were combined over experiments. However, this interaction was significant for peanut injury for Georgia (P=0.04) and North Carolina (P=0.03); therefore, data are presented by experiments. Further analysis indicated that the interaction of base herbicide by herbicide program was not significant for peanut injury and yield. Therefore, data for the main effects were combined.</p>
					<p>Peanut injury was similar from acetochlor and <italic toggle="yes">S</italic>-metolachlor at all the locations at Georgia and North Carolina except Attapulgus 2012 and Plains 2011, where greater injury was observed with <italic toggle="yes">S</italic>-metolachlor as compared to acetochlor (<xref ref-type="table" rid="i0095-3679-45-1-45-t10">Tables 10</xref> and <xref ref-type="table" rid="i0095-3679-45-1-45-t11">11</xref>). Peanut injury with respect to herbicide programs was inconsistent, and maximum injury (21%) was reported from pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST at Rocky Mount 2012 (<xref ref-type="table" rid="i0095-3679-45-1-45-t11">Table 11</xref>).</p>
					<table-wrap id="i0095-3679-45-1-45-t10" position="float" content-type="2col" orientation="portrait">
						<label>Table 10.</label>
						<caption>
							<p>Peanut injury under weedy conditions as influenced by herbicide programs containing acetochlor and <italic toggle="yes">S</italic>-metolachlor in Georgia, 2011-2012.<sup>a</sup></p>
						</caption>
						<graphic xlink:href="i0095-3679-45-1-45-t10.png" position="float" orientation="portrait"></graphic>
					</table-wrap>
					<table-wrap id="i0095-3679-45-1-45-t11" position="float" content-type="2col" orientation="portrait">
						<label>Table 11.</label>
						<caption>
							<p>Peanut injury and yield under weedy conditions as influenced by herbicide programs containing acetochlor and <italic toggle="yes">S</italic>-metolachlor in North Carolina, 2011-2012.<sup>a</sup></p>
						</caption>
						<graphic xlink:href="i0095-3679-45-1-45-t11.png" position="float" orientation="portrait"></graphic>
					</table-wrap>
					<p>There was no difference in peanut yield between acetochlor and <italic toggle="yes">S</italic>-metolachlor and peanut treated with either herbicide yielded more than the non-treated control (<xref ref-type="table" rid="i0095-3679-45-1-45-t11">Table 11</xref>). Herbicide programs including pendimethalin PRE fb lactofen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST, pendimethalin plus flumioxazin PRE fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST, pendimethalin PRE fb paraquat plus bentazon plus acifluorfen plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor EPOST fb imazapic plus either acetochlor or <italic toggle="yes">S</italic>-metolachlor POST resulted in greater yields as compared to non-treated control. The greater yield from these herbicide programs, which contain EPOST and/or POST treatments, may be attributed to effective weed management throughout the peanut growing season. Increased peanut yield has been reported in other studies by using combinations of PPI, PRE, EPOST, or POST herbicide applications (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Clewis2">Clewis <italic toggle="yes">et al</italic>., 2007</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Grichar2">Grichar and Dotray, 2012</xref>; <xref ref-type="bibr" rid="i0095-3679-45-1-45-Jordan3">Jordan <italic toggle="yes">et al</italic>., 2009</xref>).</p>
				</sec>
			</sec>
		</sec>
		<sec id="s4">
			<title>Conclusions</title>
			<p>Results from this research demonstrated that acetochlor exhibited similar phytotoxicity to peanut as <italic toggle="yes">S</italic>-metolachlor, a herbicide currently used in peanut production. This herbicide will provide growers with another option in their battle against hard-to-control weeds. In most instances, acetochlor or <italic toggle="yes">S</italic>-metolachlor are not stand-alone herbicides and should be included in a systems approach for the most effective weed control. The addition of herbicides with multiple sites of action in weed management program not only provided increased control of weeds in peanut but may also provide optimum resistance management (<xref ref-type="bibr" rid="i0095-3679-45-1-45-Heap1">Heap, 2017</xref>).</p>
		</sec>
	</body>
	<back>
		<ack>
			<title>Acknowledgments</title>
			<p>Monsanto Co. provided partial funding for this research.</p>
		</ack>
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							<surname>Prostko<x xml:space="preserve">, </x></surname></string-name>
						<string-name name-style="western">
							<given-names>W.K.</given-names>
							<surname>Vencill<x xml:space="preserve">, </x></surname></string-name><x xml:space="preserve">and </x>
						<string-name name-style="western">
							<given-names>T.M.</given-names>
							<surname>Webster  </surname></string-name>
					</person-group>
					<year>2009</year>  
					<article-title>Establishing the geographic distribution level of acetolactate synthase resistance of Palmer amaranth (<italic toggle="yes">Amaranthus palmeri</italic>) accessions in Georgia</article-title>  
					<source>Weed Technol</source>  
					<volume>23</volume><x xml:space="preserve">:</x>
					<fpage>214</fpage><x xml:space="preserve">-</x>
					<lpage>220</lpage><x xml:space="preserve">.</x>
				</element-citation>
			</ref>
		</ref-list>
			<fn-group>
                <label><p><bold>Author Affiliations</bold></p></label>
                
				<fn id="n101" fn-type="current-aff">
					<label><sup>1</sup></label>
					<p>Postdoctoral Research Scholar and Professor, Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695</p>
				</fn>
				<fn id="n102" fn-type="current-aff">
					<label><sup>2</sup></label>
					<p>Professor and Professor, respectively. Department of Crop and Soil Sciences, The University of Georgia, 2360 Rainwater Drive, Tifton, GA 31793.</p>
				</fn>
				<fn id="n103" fn-type="current-aff">
					<label><sup>3</sup></label>
					<p>Associate Professor, Department of Horticulture Science, North Carolina State University, Raleigh, NC 27695.</p>
				</fn>
				<corresp id="cor1">
					<label>*</label>Corresponding author Email: <email>david_jordan@ncsu.edu</email>
				</corresp>
			</fn-group>
	</back>
</article>
