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   <front>
      <journal-meta>
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         <journal-id journal-id-type="pmc"/>
         <journal-id journal-id-type="allenpress-id"/>
         <journal-id journal-id-type="publisher-id">pnut</journal-id>
         <journal-title-group>
            <journal-title>Peanut Science</journal-title>
         </journal-title-group>
         <issn pub-type="ppub">0095-3679</issn>
         <issn pub-type="active">0095-3679</issn>
         <publisher>
            <publisher-name>American Peanut Research and Education Society</publisher-name>
            <publisher-loc/>
         </publisher>
      </journal-meta>
      <article-meta>
         <article-id pub-id-type="other"/>
         <article-id pub-id-type="doi">10.3146/0095-3679-42.2.100</article-id>
         <article-id pub-id-type="publisher-id">0095-3679-42.2.100</article-id>
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         <article-categories>
            <subj-group subj-group-type="heading">
               <subject>ARTICLES</subject>
            </subj-group>
         </article-categories>
         <title-group>
            <article-title>Weed Control and Peanut (<italic>Arachis hypogaea</italic> L.) Cultivar Response to Encapsulated Acetochlor</article-title>
            <alt-title alt-title-type="running-head">E<sc>fficacy of</sc> A<sc>cetochlor in</sc> P<sc>eanut</sc>
            </alt-title>
         </title-group>
         <contrib-group>
            <contrib contrib-type="author" xlink:type="simple">
               <name name-style="western">
                  <surname>Grichar</surname>
                  <given-names>W. James</given-names>
                  <x xml:space="preserve"> </x>
               </name>
               <xref ref-type="aff" rid="aff1"/>
               <x xml:space="preserve">, </x>
            </contrib>
            <contrib contrib-type="author" xlink:type="simple">
               <name name-style="western">
                  <surname>Dotray</surname>
                  <given-names>Peter A.</given-names>
                  <x xml:space="preserve"> </x>
               </name>
               <xref ref-type="aff" rid="aff1"/>
               <x xml:space="preserve">, and </x>
            </contrib>
            <contrib contrib-type="author" xlink:type="simple">
               <name name-style="western">
                  <surname>Etheredge</surname>
                  <given-names>Luke M.</given-names>
                  <x xml:space="preserve"> </x>
               </name>
               <xref ref-type="aff" rid="aff1">
                  <sup>1</sup>
               </xref>
               <xref ref-type="corresp" rid="cor1">
                  <sup>*</sup>
               </xref>
            </contrib>
            <aff id="aff1">
               <label>
                  <sup>1</sup>
               </label>First author: Texas A&amp;M AgriLife Research, Corpus Christi, TX 78102; 2<sup>nd</sup> author: Texas A&amp;M AgriLife Research and Extension Service, Lubbock, TX 79403; 3<sup>rd</sup> author: Monsanto Company, Llano, TX 78643</aff>
         </contrib-group>
         
         <pub-date pub-type="ppub">
            <month>November</month>
            <year>2015</year>
         </pub-date>
         <volume>42</volume>
         <issue>2</issue>
         <fpage>100</fpage>
         <lpage>108</lpage>
         <permissions>
            <copyright-statement>© 2015 American Peanut Research and Education Society</copyright-statement>
            <copyright-year>2015</copyright-year>
         </permissions>
         <related-article related-article-type="pdf"
                          xlink:href="0095-3679-42.2.100.pdf"
                          xlink:type="simple"/>
         <abstract>
            <title>ABSTRACT</title>
            <p>Field studies were conducted during 2011 and 2012 in the Texas peanut production regions to evaluate encapsulated acetochlor for weed control and cultivar response. Acetochlor alone applied preemergence (PRE) controlled horse purslane, Palmer amaranth, smellmelon, and Texas millet as well as flumioxazin or <italic>S</italic>-metolachlor. The addition of pendimethalin to either acetochlor, flumioxazin, or <italic>S</italic>-metolachlor did improve weed control in some instances. In another study comparing the three above mentioned herbicides alone or followed by lactofen postemergence (POST), the addition of lactofen to acetochlor, flumioxazin, or <italic>S</italic>-metolachlor improved control of smellmelon and Palmer amaranth in some instances but did not affect horse purslane control. In a tolerance study to evaluate potential differences in variety response to acetochlor at 1.26 (1X) and 2.52 kg ai/ha (2X) applied preplant incorporated (PPI), PRE, early postemergence (EPOST), or POST, peanut yield or grade was not affected by either rate of acetochlor or application timing.</p>
         </abstract>
         <kwd-group>
            <title>Key Words<x xml:space="preserve">:</x>
            </title>
            <kwd>
               <italic>Amaranthus palmeri</italic> L.</kwd>
            <x xml:space="preserve">, </x>
            <kwd>
               <italic>Cucumis melo</italic> L.</kwd>
            <x xml:space="preserve">, </x>
            <kwd>
               <italic>Proboscidea louisianica</italic> L.</kwd>
            <x xml:space="preserve">, </x>
            <kwd>
               <italic>Trianthema portulacastrum</italic> L.</kwd>
            <x xml:space="preserve">, </x>
            <kwd>
               <italic>Urochloa texana</italic> (Buckl.)</kwd>
            <x xml:space="preserve">, </x>
            <kwd>groundnut</kwd>
            <x xml:space="preserve">, </x>
            <kwd>yield</kwd>
            <x xml:space="preserve">, </x>
            <kwd>grade</kwd>
            <x xml:space="preserve">.</x>
         </kwd-group>
         <counts>
            <fig-count count="0"/>
            <table-count count="4"/>
            <ref-count count="51"/>
            <page-count count="9"/>
         </counts>
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   </front>
   <body>
      <sec id="s1">
			<title>Introduction</title>
         <p>Peanut (<italic>Arachis hypogaea</italic> L.) has several unique features that contribute to challenging weed management. Peanut cultivars grown in the United States require a long growing season (140 to 160 d), depending on cultivar and geographical region [<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Henning1">Henning <italic>et al</italic>., 1982</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>., 1995</xref>]. Peanut also has a prostrate growth habit, a relatively shallow canopy, and is slow to shade inter-rows allowing weeds to be more competitive [<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Walker1">Walker <italic>et al</italic>., 1989</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>., 1995</xref>). Consequently, herbicides applied at planting may not provide season-long control and mid-to-late season weed emergence can occur. Additionally, peanut fruit develops underground on pegs originating from branches that grow along the soil surface. This prostrate growth habit and pattern of fruit development restricts cultivation to an early-season control option [<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>., 1995</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Brecke1">Brecke and Colvin, 1991</xref>). With conventional row spacing (91 to 102 cm), complete ground cover may not be attained until 8 to 10 wk after planting. In some areas of the United States peanut growing region, complete canopy closure may never occur.</p>
         <p>Weeds compete with peanut for sunlight, moisture, and nutrients and may reduce harvesting efficiency. Weeds are particularly troublesome during digging and inverting procedures (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Young1">Young <italic>et al</italic>., 1982</xref>). Weed biomass slows field-drying of peanut vines and pods and increases the likelihood of exposure to rainfall, which may increase harvesting losses (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>., 1995</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Young1">Young <italic>et al</italic>., 1982</xref>). The fibrous root system of annual grasses is extremely difficult to separate from peanut (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut3">Wilcut <italic>et al</italic>., 1994</xref>).</p>
         <p>Acetochlor is a chloroacetanilide herbicide and the mode of action is elongase inhibition and inhibition of geranylgeranyl pyrophosphate (GGPP) cyclization enzymes, which is part of the gibberellin biosynthetic pathway (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Arregui1">Arregui <italic>et al</italic>., 2010</xref>) and controls weeds by inhibiting growth of seedling shoots (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Ross1">Ross and Childs, 1996</xref>). Acetochlor controls a broad spectrum of weeds in corn (<italic>Zea mays</italic> L.), cotton (<italic>Gossypium hirsutum</italic> L.), soybean (<italic>Glycine ma</italic>x L.), and various other crops (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous2">Anonymous, 2012</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous4">2014b</xref>). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Steckel1">Steckel <italic>et al</italic>. (2002)</xref> reported inconsistent control of common waterhemp (<italic>Amaranthus rudis</italic> L.) in corn with chloroacetamide herbicides. However, they also reported that encapsulated acetochlor formulations controlled common waterhemp at least 85% regardless of application method when evaluated 56 d after planting. <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Armel1">Armel <italic>et al</italic>. (2003)</xref> reported that tank mixes of mesotrione plus acetochlor controlled smooth pigweed (<italic>Amaranthus hybridus</italic> L.) and giant foxtail (<italic>Setaria faberi</italic> Herrm.), but did not adequately control common ragweed (<italic>Ambrosia artemisiifolia</italic> L.), common lambsquarters (<italic>Chenopodium album</italic> L.), or morningglory species (<italic>Ipomoea</italic> spp.). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Geier1">Geier <italic>et al</italic>. (2009)</xref> found that acetochlor, in combination with atrazine, controlled large crabgrass [<italic>Digitaria sanguinalis</italic> (L.) Scop.] in grain sorghum [<italic>Sorghum bicolor</italic> (L.) Moench.] 55 to 76% in one yr but 94% or greater in two other yrs. However, shattercane [<italic>Sorghum bicolor</italic> (L.) Moench.] was controlled less than 20%.</p>
         <p>
            <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Parker1">Parker <italic>et al</italic>. (2005)</xref> compared acetochlor as an emulsifiable concentrate (EC) with two encapsulated formulations, capsule suspension (CS) and microencapsulated (ME). The CS formulation controlled giant foxtail 62 to 74% while the EC and ME formulations controlled 43 to 46%, 180 d after application. The encapsulated formulation of acetochlor (Warrant®) (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous1">Anonymous, 2010</xref>) is now commercially available and provides greater crop safety in several crops, including soybean and was designed to give PRE and postemergence (POST) weed control in acetolactate synthase (ALS) and glyphosate resistant weeds (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous1">Anonymous, 2010</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous4">2014b</xref>). The encapsulated formulation requires limited moisture for activation (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous1">Anonymous, 2010</xref>), helps minimize crop injury, and also can extend weed control for up to 40 d (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous1">Anonymous, 2010</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous4">2014b</xref>). Acetochlor recently received approval from the Environmental Protection Agency for use on peanut in the U. S. (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous4">Anonymous, 2014b</xref>).</p>
         <p>The objectives of this research were: 1) to evaluate and compare weed efficacy of the new encapsulated acetochlor with flumioxazin, <italic>S</italic>-metolachlor, and pendimethalin alone or acetochlor in combination with pendimethalin or followed by POST applications of lactofen, and 2) to evaluate peanut cultivar response to the encapsulated acetochlor when applied at different rates and timings.</p>
      </sec>
      <sec id="s2">
         <title>Materials and Methods</title>
         <p>Studies were conducted during 2011 and 2012 at Texas A&amp;M AgriLife Research site near Yoakum in south-central Texas (29.276<sup>o</sup> N, 97.123<sup>o</sup> W) and at the Texas A&amp;M AgriLife Research and Extension Center at Halfway (34.188<sup>o</sup> N, 101.952<sup>o</sup> W) in the Texas High Plains. Soil at the Yoakum site was a Tremona loamy fine sand (thermic Aquic Arenic Palenstalf) with less than 1% organic matter and pH 7.2, while the soil at Halfway was a Pullman clay loam (fine, mixed, thermic Torrertic Paleustoll) with less than 1% organic matter and pH 7.7.</p>
         <sec id="s2a">
            <title>Weed Efficacy Studies</title>
            <p>For the first weed efficacy study (Study 1), experimental design was a randomized complete block with 3 replications at Yoakum and 3 or 4 replications at the Halfway location. Plot dimensions at Yoakum were two rows (spaced 97 cm apart) by 7.9 m long and four rows (spaced 102 cm apart) by 9.5 m long at Halfway. Experimental sites contained natural infestations of devil’s-claw (at Halfway, densities of 2 to 3 plants/m<sup>2</sup>), horse purslane (at Yoakum, densities were 5 to 7 plants/m<sup>2</sup>), Palmer amaranth (densities at Yoakum were 1 to 2 plants/m<sup>2</sup> while at Halfway populations were greater than 2 plants/m<sup>2</sup>), smellmelon (at Yoakum, denisities of 4 to 6 plants/m<sup>2</sup>), and Texas millet (at Yoakum, densities of 2 to 4 plants/m<sup>2</sup>).</p>
            <p>Herbicide treatments included acetochlor, flumioxazin, pendimethalin, or <italic>S</italic>-metolachlor alone at 1.28, 0.11, 1.06, or 1.46 kg ai/ha, respectively, applied preemergence (PRE). Pendimethalin plus flumioxazin, pendimethalin plus <italic>S</italic>-metolachlor, and pendimethalin plus acetochlor were applied PRE in combination at the above mentioned rates. Pendimethalin applied PRE was followed by postemergence (POST) applications of either imazapic alone at 0.07 kg ai/ha, lactofen alone at 0.22 kg ai/ha, lactofen plus <italic>S</italic>-metolachlor or lactofen plus acetochlor at the above mentioned rates. Postemergence applications of imazapic, lactofen, lactofen plus <italic>S</italic>-metolachlor, or lactofen plus acetochlor included a crop oil concentrate (Agridex, Helena Chemical Co., Memphis, TN 38119) at 1% (v/v) at both locations. The pendimethalin followed by imazapic treatment was included because it is a commercial standard in many peanut growing areas of the state (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar 2007</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">2008</xref>). An non-treated check was also included at both locations. Peanut yield was taken at the Halfway location but not Yoakum due to high weed density which made digging difficult (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Buchanan1">Buchanan <italic>et al</italic>., 1982</xref>).</p>
            <p>For the second weed efficacy study (Study 2), conducted at Yoakum and Halfway, the experimental design was a factorial arrangement with a randomized complete block design and herbicide treatments were replicated 3 times. Treatments consisted of a factorial arrangement of PRE herbicide treatments (acetochlor at 1.26 kg/ha, flumioxazin at 0.11 kg/ha, and <italic>S</italic>-metochlor at 1.46 kg/ha) and POST herbicide (no POST herbicide or lactofen at 0.22 kg/ha). Lactofen treatments included a crop oil concentrate (Agridex, Helena Chemical Co., Memphis, TN 38119) at 1% (v/v) at both locations. A non-treated check was included at both locations. Row configurations and weed populations were similar to those mentioned in Study 1. Peanut yield was not collected at either location due to difficulty of digging plots with high weed populations as mentioned earlier (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Buchanan1">Buchanan <italic>et al</italic>., 1982</xref>).</p>
            <p>Preemergence herbicides were applied within 24 h after peanut planting. Rainfall or irrigation followed within 7 to 14 d to activate PRE herbicides. Postemergence herbicides were applied 3 to 4 wks after planting at Yoakum or 6 to 8 wks after planting at Halfway and were applied when devil’s-claw, Palmer amaranth, Texas millet, or horse purslane were less than 42 cm in height while smellmelon was less than 30 cm in length.</p>
         </sec>
         <sec id="s2b">
            <title>Peanut Tolerance Studies</title>
            <p>In the peanut tolerance studies (Study 3), plots were kept weed-free using a combination of hand hoeing or POST herbicides which did not impact crop growth and development. Clethodim at 0.11 kg ai/ha, lactofen at 0.22 kg/ha, or 2,4-DB at 0.28 kg ae/ha were applied with a crop oil concentrate (Agridex) to control annual grasses and broadleaf weeds, respectively.</p>
            <p>Field studies were conducted at Yoakum in 2011 and 2012 and at Halfway in 2012. At Yoakum, three runner market type cultivars were evaluated, ‘Tamrun OL01’ (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Simpson2">Simpson <italic>et al</italic>., 2003b</xref>) was planted both years while ‘Tamrun OL07’ (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Baring1">Baring <italic>et al</italic>., 2006</xref>) was planted in 2011 and ‘McCloud’ (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anonymous3">Anonymous, 2014a</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Beasley1">Beasley and Baldwin, 2009</xref>) was planted in 2012. At Halfway, the Spanish market type, ‘OLin’ (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Simpson1">Simpson <italic>et al</italic>., 2003a</xref>) peanut was planted in 2012. Herbicide treatments consisted of a factorial arrangement of herbicide treatments (acetochlor at 1.26 and 2.52 kg/ha, <italic>S</italic>-metochlor at 1.46 kg/ha) and application timings [preplant incorporated (PPI), PRE, early postemergence (EPOST), and POST].</p>
         </sec>
         <sec id="s2c">
            <title>Variables for All Studies</title>
            <p>Herbicides were applied in water using a CO<sub>2</sub>-pressurized backpack sprayer with TeeJet® 11002 DG (Spraying Systems Company, P.O. Box 7900, North Avenue, Wheaton, IL 60188) nozzles calibrated to deliver 190 L/ha at 180 kPa at Yoakum and TurboTee® 11002 nozzles in 2011 and TurboTee® 11003 calibrated to deliver 190 L/ha in 2011 or 140 L/ha in 2012 at 207 kPa at Halfway.</p>
            <p>At Yoakum, PPI herbicides were incorporated immediately after application with a power-driven rotary tiller to a depth of approximately 6 cm while at the Halfway location herbicides were incorporated with a field cultivator, set to a depth of approximately 6 cm. Preemergence herbicides were applied within 24 h after peanut planting. Rainfall or irrigation followed within 7 to 14 d to activate PRE herbicides. Early POST herbicides were applied approximately 3 wks after peanut planting at Yoakum and 8 wks after planting at Halfway.</p>
            <p>Peanut were planted at Yoakum using a Monosem® vacuum planter calibrated to plant 170,000 seed/ha while at Halfway a John Deere® JD 1700 Series MaxEmerge 4-row planter calibrated to plant 160,000 (2011) to 210,000 (2012) seed/ha was used. Planting depth was approximately 4 to 5 cm at both locations.</p>
            <p>Weed control or peanut injury was estimated visually using a scale of 0 (no weed control or peanut injury) to 100 (complete weed control or plant death) (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Frans1">Frans <italic>et al</italic>., 1986</xref>). Weed control ratings were taken 28 to 150 d after peanut were planted depending on location while peanut injury (chlorosis/stunting) was rated 14 to 28 d after PRE herbicide application or 5 to 7 d after POST herbicide application.</p>
            <p>Peanut yields were obtained by inverting each plot separately, air-drying in the field for 4 to 7 d, and harvesting peanut pods from each plot with a combine. Weights were recorded after soil and trash were removed from plot samples. Grade samples were determined using screens specified in USDA grading procedures (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-DeptofAgricultureUSDA1">USDA, 1998</xref>).</p>
            <p>Visual estimates of weed control and peanut injury were arcsine square root transformed prior to analysis of variance, but are expressed in their original form for clarity because the transformation did not alter interpretation. Means were compared with Fisher’s Protected LSD test at the 5% probability level (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-SAS1">SAS Institute Inc., 2007</xref>). The non-treated check was not included in the weed control or peanut injury analysis but was included in peanut yield and grade analysis.</p>
         </sec>
      </sec>
      <sec id="s3">
         <title>Results and Discussion</title>
         <sec id="s3a">
            <title>Peanut Injury</title>
            <p>No peanut injury was observed with any PRE herbicides (data not shown). Many growers have reported peanut stunting when PPI or PRE applications of metolachlor have been followed by rain (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar1">Grichar <italic>et al</italic>., 1996</xref>). They also reported that POST applications of metolachlor followed by irrigation within 24 h could be effective for yellow nutsedge (<italic>Cyperus esculentus</italic> L.) control and reduce the chance of peanut injury from soil applications of metolachlor. Combinations of factors, such as herbicide rate, moisture conditions at planting, soil organic matter, and pH may affect peanut injury by chloroacetamide herbicides, such as acetochlor and <italic>S</italic>-metolachlor (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Cardina1">Cardina and Swann, 1988</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Mueller1">Mueller <italic>et al</italic>., 1999</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Osborne1">Osborne <italic>et al</italic>., 1995</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wehtje1">Wehtje <italic>et al</italic>., 1988</xref>). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Cardina1">Cardina and Swann (1988)</xref> reported that metolachlor often delayed peanut emergence and reduced peanut growth when irrigation followed planting. However, yield loss was observed only when metolachlor was applied at rates three times higher than recommended by the manufacturer.</p>
            <p>Flumioxazin can injure peanut, especially when the application is delayed until peanut emergence (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Johnson1">Johnson <italic>et al</italic>., 2006</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Jordan1">Jordan <italic>et al</italic>., 2009</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Tredaway-Ducar1">Tredaway-Ducar <italic>et al</italic>., 2009</xref>). When applied soon after peanut planting (1 to 2 d), <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar2">Grichar <italic>et al</italic>. (2004)</xref> reported that flumioxazin plus metolachlor combinations, under cool, wet conditions resulted in peanut stunting which was evident throughout the growing season. Injury was attributed to increased absorption of flumioxazin and metolachlor with the heavy rainfall and the slowed metabolism of these herbicides as a result of cool temperatures (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Yoshida1">Yoshida <italic>et al</italic>., 1991</xref>). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Askew1">Askew <italic>et al</italic>. (1999)</xref> reported that flumioxazin at 0.07 and 0.11 kg/ha injured peanut 45 and 62%, respectively, when evaluated 2 wks after peanut planting. Peanut stunting of greater than 60% was followed by as much as 35% leaflet discoloration, which was characterized as necrotic spots on foliage. <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Scott1">Scott <italic>et al</italic>. (2001)</xref> reported that flumioxazin treated peanuts were injured 10% when evaluated 3 wks after planting. However, injury was transient and was not apparent 6 wk after planting. Flumioxazin enters plants mainly by shoot and root uptake, and plant injury can be mitigated by rapid metabolism (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Yoshida1">Yoshida <italic>et al</italic>., 1991</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Anderson1">Anderson <italic>et al</italic>., 1994</xref>).</p>
            <p>Leaf chlorosis and necrosis with lactofen never exceeded 15% 5 to 7 d after POST herbicide application (data not shown). This injury was evident for several wks after application on older tissue. Subsequent new growth did not show the effects of the lactofen applications and was 2% or less four wks after application (data not shown). Other studies have reported that lactofen injury is transient and subsequent growth does not show any effects of the herbicide (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar, 2007</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">2008</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray, 2011</xref>).</p>
         </sec>
         <sec id="s3b">
            <title>Weed Control</title>
            <sec id="s3b1">
               <title>Study 1</title>
               <sec id="s3b1a">
                  <title>Palmer amaranth</title>
                  <p>Since there was no treatment by year interaction at Yoakum for control of this weed, data were combined over years; at the Halfway location data are presented by year due to a treatment by year interaction.</p>
                  <p>At Yoakum, under low weed pressure, there was no difference in control between any of the herbicide treatments with control exceeding 90%. At the Halfway location in 2011, <italic>S</italic>-metolachlor alone and pendimethalin plus either <italic>S</italic>-metolachlor or acetochlor applied PRE controlled Palmer amaranth at least 93%, while pendimeathlin or flumioxazin alone provided less than 63% control (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t01">Table 1</xref>). Pendimethalin applied PRE followed by POST applications of either imazapic or lactofen plus <italic>S</italic>-metolachlor controlled Palmer amaranth at least 94%. In 2012, either pendimethalin alone or pendimethalin plus lactofen combinations provided 44 to 73% control.</p>
                  <table-wrap id="pnut-0095-3679-42.2.100-t01"
                              content-type="12col"
                              position="float">
                     <label>Table 1. </label>
                     <caption>
                        <p>Comparison of weed control programs with acetochlor, flumioxazin, imazapic, <italic>S</italic>-metochlor, lactofen, and pendimethalin in south Texas and the High Plains of Texas (Study 1).<sup>a</sup>
                        </p>
                     </caption>
                     <graphic xlink:href="0095-3679-42.2.100-t01.png"
                              mimetype="image"
                              position="float"
                              xlink:type="simple"/>
                  </table-wrap>
                  <p>Monoculture production systems and the repeated use of herbicides with the same mode of action have led to herbicide resistance in weeds, especially Palmer amaranth (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Culpepper1">Culpepper <italic>et al</italic>. 2006</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Peterson1">Peterson, 1999</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-VanGessel1">VanGessel, 2001</xref>). The use of soil-applied and POST herbicides with alternative modes of action are recommended to delay the rate of development of herbicide-resistant weed populations (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Shaner1">Shaner <italic>et al</italic>., 1997</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Ellis1">Ellis and Griffin, 2002</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Craigmyle1">Craigmyle <italic>et al</italic>. 2013</xref>).</p>
               </sec>
               <sec id="s3b1b">
                  <title>Smellmelon</title>
                  <p>There was a treatment by year interaction at Yoakum so data are presented separately by year. In 2011, smellmelon control with either acetochlor, flumioxazin, pendimethalin, or <italic>S</italic>-metolachlor alone was less than 70% while pendimethalin applied PRE followed by either imazapic or lactofen combinations applied POST provided at least 97% control (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t01">Table 1</xref>). In 2012, smellmelon control with acetochlor, flumioxazin, pendimethalin, or <italic>S</italic>-metolachlor alone ranged from 67 to 75% while all pendimethalin combinations provided 86 to 99% control. <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar6">Grichar and Dotray (2013)</xref> reported inconsistent control of smellmelon with flumioxazin. In one year, flumioxazin at either 0.07 or 0.11 kg/ha controlled less than 55% while in another year smellmelon control was 77 and 96%, respectively.</p>
               </sec>
               <sec id="s3b1c">
                  <title>Texas millet</title>
                  <p>Since there was a treatment by year interaction at Yoakum, each year is presented separately. Acetochlor, flumioxazin, <italic>S</italic>-metolachlor, and pendimethalin alone controlled this weed at least 90% while pendimethalin plus either flumioxazin or acetochlor applied PRE or followed by imazapic applied POST provided at least 97% control during 2011 (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t01">Table 1</xref>). In 2012, flumioxazin and pendimethalin alone controlled this weed 85 and 93%; respectively, while acetochlor and <italic>S</italic>-metolachlor provided 67 to 77% control. All pendimethalin combinations with the exception of pendimethalin plus acetochlor applied PRE or pendimethalin followed by lactofen plus <italic>S</italic>-metolachlor applied POST controlled Texas millet at least 92%.</p>
                  <p>
                     <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>. (1995)</xref> reported that metolachlor provided little or no Texas millet control. In contrast, the dinitroaniline herbicides provide excellent control of annual grasses (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Buchanan1">Buchanan <italic>et al</italic>., 1982</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Chamblee1">Chamblee <italic>et al</italic>., 1982</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut3">Wilcut <italic>et al</italic>., 1994</xref>) including full-season control of Texas millet (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut1">Wilcut <italic>et al</italic>., 1987a</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut2">1987b</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">1995</xref>).</p>
               </sec>
               <sec id="s3b1d">
                  <title>Horse purslane</title>
                  <p>This weed was present at Yoakum only in 2011. All herbicide systems controlled this weed at least 80% and the addition of imazapic or lactofen applied POST provided complete control (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t01">Table 1</xref>). Imazapic typically does not control horse purslane (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar, 2007</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">2008</xref>). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar (2007)</xref> reported that pendimethalin, flumioxazin, imazethapyr, <italic>S</italic>-metolachlor, or dimethenamid-P provided 73% control of horse purslane when used alone.</p>
               </sec>
               <sec id="s3b1e">
                  <title>Devil’s-claw</title>
                  <p>This weed was present at Halfway only in 2011. Pendimethalin applied PRE followed by imazapic applied POST provided acceptable control (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t01">Table 1</xref>). Combinations that included lactofen controlled this weed no better than 63%. <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray (2011)</xref> reported that lactofen alone failed to control devil’s-claw but a sequential application of lactofen followed by 2,4-DB controlled at least 88%.</p>
               </sec>
               <sec id="s3b1f">
                  <title>Peanut yield</title>
                  <p>In 2011, there was no difference in yield between any of the herbicide treatments and the non-treated check (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t02">Table 2</xref>). In 2012, pendimethalin plus acetochlor applied PRE produced the greatest yields while the non-treated check produced the lowest. Competition from Palmer amaranth can severely reduce peanut yield (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar, 2007</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">Grichar, 2008</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut1">Wilcut, <italic>et al</italic>. 1987a</xref>). Not only does the competition from these weeds reduce peanut yield but their extensive root system interferes with harvesting efficiency (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Buchanan1">Buchanan <italic>et al</italic>., 1982</xref>).</p>
                  <table-wrap id="pnut-0095-3679-42.2.100-t02"
                              content-type="7col"
                              position="float">
                     <label>Table 2. </label>
                     <caption>
                        <p>Peanut response to acetochlor under weedy conditions in the High Plains of Texas (Study 1).</p>
                     </caption>
                     <graphic xlink:href="0095-3679-42.2.100-t02.png"
                              mimetype="image"
                              position="float"
                              xlink:type="simple"/>
                  </table-wrap>
               </sec>
               <sec id="s3b1g">
                  <title>Peanut grade</title>
                  <p>In 2011, pendimethalin applied PRE followed by lactofen plus <italic>S</italic>-metolachlor produced the lowest grade (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t02">Table 2</xref>). In 2012, pendimethalin applied PRE followed by lactofen plus acetochlor applied POST produced the lowest grade while acetochlor and <italic>S</italic>-metochlor alone and pendimethalin plus either flumioxazin or <italic>S</italic>-metolachlor produced the highest.</p>
               </sec>
            </sec>
            <sec id="s3b2">
               <title>Study 2</title>
               <sec id="s3b2a">
                  <title>Smellmelon</title>
                  <p>There was a treatment by year interaction; therefore, each year is presented separately. When rated 21 d after PRE applications, only flumioxazin provided moderately acceptable control (75 to 81%) in 2011 while in 2012, all PRE herbicides alone controlled smellmelon at least 96% (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t03">Table 3</xref>). In 2012, 1.9 mm of rain fell within 4 d of PRE herbicide application and this accounted for the excellent control with all PRE herbicides. Since the PRE herbicides can photodegrade on the soil surface, these herbicides need to be mechanically incorporated or activated by rainfall or irrigation (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">Wilcut <italic>et al</italic>., 1995</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar1">Grichar <italic>et al</italic>., 1996</xref>) which explains the erratic control noted between the two years. When rated 56 d after PRE application in 2011, flumioxazin, <italic>S</italic>-metolachlor, or acetochlor alone failed to adequately control smellmelon while the addition of lactofen applied POST improved control to at least 98% for all combinations. In 2012 <italic>S</italic>-metolachlor alone provided 95% control and the addition of lactofen to the PRE herbicides provided at least 94% smellmelon control. In previous work, <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">Grichar (2008)</xref> and <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray (2011)</xref> reported that smellmelon control with lactofen varied from 66 to 88% regardless of application timing. Also, <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray (2011)</xref> reported that lactofen controlled smellmelon 2 to 5 cm long at least 97% with either Agridex or Induce; however, smellmelon 15 to 20 cm long was controlled 96% with lactofen plus Agridex but only 82% with lactofen plus Induce. They also reported that high smellmelon densities in plots with poor smellmelon control can result in the smellmelon vines as well as the tight fibrous root system of the plant becoming intertwined with the peanut plant and digging equipment during the digging operation. As a result, many peanut pods can be stripped from the peanut plant during the digging process.</p>
                  <table-wrap id="pnut-0095-3679-42.2.100-t03"
                              content-type="13col"
                              position="float">
                     <label>Table 3. </label>
                     <caption>
                        <p>Comparison of weed control programs with acetochlor, flumioxazin, or <italic>S</italic>-metolachlor alone or followed by lactofen in south Texas and the High Plains of Texas (Study 2).<sup>a</sup>
                        </p>
                     </caption>
                     <graphic xlink:href="0095-3679-42.2.100-t03.png"
                              mimetype="image"
                              position="float"
                              xlink:type="simple"/>
                  </table-wrap>
               </sec>
               <sec id="s3b2b">
                  <title>Horse purslane</title>
                  <p>This weed was present at Yoakum only in 2011. All herbicide systems controlled this weed at least 89% at either rating date (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t03">Table 3</xref>). <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar3">Grichar (2007)</xref> and <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray (2011)</xref> reported that lactofen alone controlled horse purslane at least 88% regardless of application timing.</p>
               </sec>
               <sec id="s3b2c">
                  <title>Palmer amaranth</title>
                  <p>This weed was present at Yoakum only in 2011 and at Halfway in both years. At Halfway, early season (43 d after PRE treatment) ratings were combined over years due a lack of treatment by year interaction while later season (95 and 150 d after PRE treatment) ratings are presented separately due to a treatment by year interaction.</p>
                  <p>At Yoakum, under low weed pressure (1 to 2 plants/m<sup>2</sup>), all herbicide systems provided nearly complete control when rated either 28 d or 56 d after PRE application with the exception of <italic>S</italic>-metolachlor alone which controlled this weed 80% (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t03">Table 3</xref>). At Halfway, when rated 43 d after PRE application, all herbicide systems provided 100% control. In 2011, when rated 150 d after PRE application, acetochlor or <italic>S</italic>-metolachlor either alone or followed by lactofen applied POST controlled Palmer amaranth 70 to 87% (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t03">Table 3</xref>). In 2012, when rated 95 d after PRE application, all systems provided at least 93% control with the exception of acetochlor or flumioxazin alone which controlled this weed 85 and 87%, respectively.</p>
                  <p>In previous work, pendimethalin alone controlled Palmer amaranth less than 42% while flumioxazin alone at 0.07 and 0.11 kg/ha provided 72 and 85% control, respectively (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">Grichar, 2008</xref>). In addition, lactofen applied POST following pendimethalin applied PPI improved Palmer amaranth control to 100%. Pigweed spp. can effectively be controlled with the dinitroaniline herbicides (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut3">Wilcut <italic>et al</italic>., 1994</xref>). Metolachlor applied PPI or PRE controls pigweed less consistently than dinitroaniline herbicides (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut3">Wilcut <italic>et al</italic>., 1994</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Wilcut4">1995</xref>).</p>
               </sec>
            </sec>
         </sec>
         <sec id="s3c">
            <title>Peanut Cultivar Response</title>
            <sec id="s3c1">
               <title>Study 3</title>
               <p>At Yoakum, only a cultivar response was noted in one of two years. No differences were noted in 2011; however, in 2012, McCloud produced a higher yield that Tamrun OL01 (<xref ref-type="table" rid="pnut-0095-3679-42.2.100-t04">Table 4</xref>). No difference in grade of sound mature kernels plus sound splits (SMK+SS) between cultivars was noted at the Yoakum location. At Halfway, no effect of any herbicide treatment or application timing was noted on peanut yield or grade (data not shown). With the Olin variety, yields in 2011 ranged from 2500 kg/ha for the untreated to 2950 kg/ha for acetolachlor at 1.26 kg/ha applied PRE. Grades ranged from 64.2% for the non-treated control to 67.9% for acetochlor at 2.52 kg/ha applied PPI (data not shown).</p>
               <table-wrap id="pnut-0095-3679-42.2.100-t04"
                           content-type="5col"
                           position="float">
                  <label>Table 4. </label>
                  <caption>
                     <p>Peanut variety response in 2011 and 2012 at Yoakum (Study 3).</p>
                  </caption>
                  <graphic xlink:href="0095-3679-42.2.100-t04.png"
                           mimetype="image"
                           position="float"
                           xlink:type="simple"/>
               </table-wrap>
            </sec>
         </sec>
      </sec>
      <sec id="s4">
         <title>Conclusions</title>
         <p>Results from this research demonstrated that acetochlor controlled weeds similar to several herbicides currently used in peanut production without any phytotoxicity to peanut. In most instances, acetochlor is not a stand-alone herbicide and should be included in a systems approach for the most effective weed control. This herbicide will provide growers with another option in their arsenal against hard-to-control weeds. In the past, some growers in the southwest suggested that a total POST program using only imazapic or imazethapyr would be sufficient; however, a soil-applied herbicide is important in order to maintain season-long weed control, increase net returns, and avoid herbicide resistant issues (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar4">Grichar, 2008</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Grichar5">Grichar and Dotray, 2011</xref>). Monoculture production systems and the repeated use of herbicides with similar modes of action have led to herbicide resistance in weeds (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Culpepper1">Culpepper <italic>et al</italic>., 2006</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Peterson1">Peterson, 1999</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-VanGessel1">VanGessel, 2001</xref>; <xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Lovell1">Lovell <italic>et al</italic>., 1999</xref>). Since <italic>Amaranthus</italic> spp. are sensitive to ALS-inhibiting herbicides and possess characteristics that predispose them to have herbicide resistant biotypes, the use of soil-applied and POST herbicides with alternative sites of action are necessary to reduce the rate of development of herbicide-resistant weed populations (<xref ref-type="bibr" rid="pnut-0095-3679-42.2.100-Shaner1">Shaner <italic>et al</italic>., 1997</xref>).</p>
      </sec>
   </body>
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                    <label>
                        <sup>1</sup>
                    </label>First author: Texas A&amp;M AgriLife Research, Corpus Christi, TX 78102; 2<sup>nd</sup> author: Texas A&amp;M AgriLife Research and Extension Service, Lubbock, TX 79403; 3<sup>rd</sup> author: Monsanto Company, Llano, TX 78643
                </fn>
                
            <corresp id="cor1">
               <label>*</label>Corresponding author: W. James Grichar, <email xlink:type="simple">w-grichar@tamu.edu</email>
            </corresp>
         </fn-group>
   </back>
</article>
